Regulatory

Part:BBa_K5223999:Design

Designed by: Junxian Li   Group: iGEM24_SCU-China   (2024-09-30)


High-intensity 35S promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First, we used TSSPlant to predict the transcription start sites (TSS) corresponding to the wild-type promoter sequences, extracting the -165 to +5 fragments relative to the TSS and performing random single-point mutations on them. The model was then used to predict the strength of the mutants, selecting the ones with the highest strength for the next round of evolution. Ultimately, through 10 and 15 rounds of evolution, we obtained mutants 1 and 2, with predicted strengths of 3.32 times and 2.08 times that of the wild type, respectively.


Source

Cauliflower Mosaic Virus (CaMV)

References